Difference between revisions of "DNA Submission"

Revision as of 14:24, 29 July 2015

Send a Part Sample to the Registry

You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it.

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Mailing Address

iGEM Foundation

Attn: Meagan Lizarazo

One Kendall Square

Suite B6104

Cambridge, MA 02139

USA

hq@igem.org

+1-617-500-3106

iGEM 2015 Requirements:

iGEM 2015 teams, remember that the deadline for shipping your parts for the 2015 competition is September 18. Your submission form must include an accurate tracking number showing a send date on or before September 18th.

Preparing your DNA Shipment

The Registry accepts miniprepped plasmid DNA (BioBrick RFC10 compatible part samples in pSB1C3, at least 10ul of 25ng/ul, (250ng total when dried down) in 96-well plates, now provided in the Submission Kit. Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.

See the pages below, for more in-depth information on requirements, submission form instructions, and how to prepare your physical shipment.

Things to keep in mind...

Documented

Parts shipped to the Registry must be documented on the Registry.
• You must add your part to the Registry; it will then receive a part name (BBa_...)
• Your part's sequence must be entered into the Registry
• Annotate your part's sequence with features, add information on how your part works and how to use it best, and include characterization and measurement data
• Quality over quantity: sending a few well-documented parts is always better than sending several that are not
• For examples check out the collection of well documented parts.

Assembly compatible

Parts must be BioBrick (RFC 10) or iGEM Type IIS compatible
• The Registry can test and maintain all parts in the same way
• Your part can be assembled to others.
• Users can easily move/assemble the part into another plasmid backbone
• Check if your part is BioBrick or Type IIS compatible by looking at the part's sequence and feature box.
• Remember, iGEM and the Registry is built on standard biological parts!

pSB1C3

Samples must be in pSB1C3, the Registry's standard shipping backbone
• Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
• The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
• pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
• Users can easily move/assemble the part into another plasmid backbone
• You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.

DNA Submission

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Sample submission of parts is no longer a requirement for the iGEM competition.

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Requirements

Here's how part samples are sent to the Registry

Why pSB1C3?

Why do samples need to be shipped in pSB1C3?

Detailed Instructions

Instructions on how to fill out the Registry's submission form.

Submission Prep

Registry groups must follow iGEM's submission prep guidelines.

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Have questions on requirements or shipping part samples? Check the FAQ or contact us at hq (at) igem . org .

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Frequently Asked Questions: Submission Requirements

What are the requirements for submitting a part sample to the Registry?

The requirements for submission...

• The part must be added and documented on the Registry.
• The part must be BioBrick RFC10 or iGEM Type IIS compatible.
• The part sample must be in pSB1C3, the Registry's shipping plasmid backbone.
• A completed submission form.
• Parts must be prepared in the correct submission format.

Please see the Registry's submission requirements page for more information.

Why do parts sent to the Registry need to be BioBrick RFC10 or Type IIS compatible

iGEM and the Registry are built on standard parts which is vital to making biology easier (and more predictable) to engineer, for high school teams & university teams to research labs. It is also why iGEM has made BioBrick RFC10 and iGEM Type IIS compatibility a requirement; the parts that we receive from teams and labs can be used and assembled by everyone easily and reliably, without the need to substantially alter, experiment, or research alternate methods.

Does the Registry allow other Assembly Standards for parts?

For iGEM teams, only BioBrick RFC10 and iGEM Type IIS compatible parts will fulfill medal requirements and be eligible for awards.

However, you can add parts that aren't compatible with those two standards to the Registry. The Registry supports multiple assembly standards and is also open to integrating new ones. You can easily see what Registry supported assembly standards your part is compatible with, by taking a look at the Sequence & Features box.

My part is not BioBrick compatible

Before working with your part in the lab (characterizing/measuring), make sure it is BioBrick compatible. If not...

• Synthesize your part to remove any illegal restriction sites, through synonymous substitutions (silent mutations).
  • Synthesis is also useful, since you can synthesize your part with the BioBrick prefix and suffix, so it is ready for shipping to the Registry. See our synthesis offer with IDT
• Use site-specific mutagenesis to remove any illegal restriction sites, through synonymous substitutions (silent mutations).

Currently, the Registry only accepts parts samples that are BioBrick compatible. If you have questions about this requirement, please don't hesitate to contact iGEM HQ (hq (at) igem . org)! Contact us early and we may be able to address your concerns.

Why do my parts need to be submitted in pSB1C3

Having a shipping standard backbone lets the Registry receive and handle all parts in the same way: using the same growth conditions and quality control measures. With over 1000 new parts submitted each year, this improves our ability to handle, maintain, and test new parts. When anyone uses the part, they'll know exactly what to expect. They can easily unpackage it from the shipping plasmid backbone, and transfer it into a different backbone for a specific use.

Shipping in pSB1C3 ensures...

• Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
• users can assemble these parts with 3A Assembly to build new devices and projects
• The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
• pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts
• part samples can be easily moved from one plasmid backbone to another (for shipping, measurement, operation etc.)

My part cannot be submitted in pSB1C3 because of ______

First, contact iGEM HQ (hq (at) igem . org)! Contact us early and we may be able to address your concerns.

There may be a reason why a part sample cannot be submitted in pSB1C3. For example...

• The part contains chloramphenicol resistance. Contact iGEM HQ first. You must use pSB1K3 instead.
• The part is toxic to cells in a high copy plasmid backbone. Contact iGEM HQ first. You must use pSB3C5 instead.

My part is not meant to be used/operated in pSB1C3, can I use ______ instead

Unfortunately, no. Please remember, that the shipping standard backbone lets the Registry receive and handle all parts in the same way. In turn, when these samples are distributed to users, they'll know exactly what to expect. They can easily unpackage it from the shipping plasmid backbone, and transfer it into a different backbone for a specific use.

We've created a new plasmid backbone. How can we submit it to the Registry?

While the Registry does not specialize in plasmids, you can send your plasmid backbone with BBa_J04450, the Registry's default insert. Your plasmid backbone must be able to replicate in E. coli.

Please contact the Registry prior to plasmid backbone submission.

If you are an iGEM team planning on nominating a plasmid backbone for medals/awards, please be aware that the judging form system is looking specifically for parts.

Can we send part samples after the deadline?

There is no longer a shipping deadline for part samples for the 2019 Competition.

If you're an iGEM Lab, you can send part samples at anytime.