Difference between revisions of "Part:BBa F2620"
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Revision as of 23:03, 3 October 2006

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: 0.1 - 1000 nM 3OC6HSL, exogenous
Output Swing: 13.6 - 490.2 GFP molecules/s.cfu
Switch Point: 6 nM 3OC6HSL, exogenous
L/H Response time: 25 mins
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator
Transfer Function
 Specificity
Response time
 Stability
Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: 92/74 generations (Low/High Input)
Performance Stability: 92/74 generations (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmid: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

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