Difference between revisions of "Part:BBa J45400:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(3 intermediate revisions by 2 users not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3.  So...don't use EcoRI or SpeI when making assemblies with this part!
+
*See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
−
[[Image:IAGDActual.jpg]]
+
*<partinfo>BBa_J45009</partinfo> has BioBrick restriction sites EcoRI and SpeI.  Thus, <partinfo>BBa_J45400</partinfo> can only be used as a suffix part.
  
 
===Source===
 
===Source===
−
 
+
<partinfo>BBa_R0011</partinfo> and <partinfo>BBa_J45398</partinfo>.
−
The coding regions (BAT2 and THI3) in this part came from S. cerevisae.
+
  
 
===References===
 
===References===
 +
See [[Part:BBa_J45008:Design#References]] and [[Part:BBa_J45009:Design#References]].

Latest revision as of 20:25, 25 March 2008

3-methylbutanal generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
  • BBa_J45009 has BioBrick restriction sites EcoRI and SpeI. Thus, BBa_J45400 can only be used as a suffix part.

Source

BBa_R0011 and BBa_J45398.

References

See Part:BBa_J45008:Design#References and Part:BBa_J45009:Design#References.