Coding
THI3
Part:BBa_J45009:Design
Designed by: Veena Venkatachalam Group: iGEM06_MIT (2006-06-07)
ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
- Constructed by PCR
Template
Saccharomyces cerevisiae strain ACLY387 genome
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA Gat gaa ttc tag cta tac aca gag -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAg tat cca act tga ttt ttt ttt aga ag -3'
Source
- THI3 from Saccharomyces cerevisiae (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=1431099&from=520&to=2349&view=gbwithparts Z74128 REGION: 520..2349])
- [http://db.yeastgenome.org/cgi-bin/locus.pl?sgdid=S000002238 THI3 SGD entry]
References
Refers only indirectly to THI3 Dickinson-JBC-1997. <biblio>
- Dickinson-JBC-1997 pmid=9341119
- Yoshimoto-ApplMicrobiolBiotechnol-2002 pmid=12172617
- Nishimura-1992 pmid=1624458
- Lilly--2006 pmid=16879424
</biblio>