Coding
BAT2
Part:BBa_J45008:Design
Designed by: Veena Venkatachalam Group: iGEM06_MIT (2006-06-07)
branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1030
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
- Constructed by PCR
Template
Saccharomyces cerevisiae strain ACLY387 genome
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA Gat gac ctt ggc acc cct aga cg -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAg ttc aaa tca gta aca acc -3'
Forward mutagenesis primer
5'- CGG GCA AGA AGG AAC TGG TTA CTG CTC CAC TAG -3'
Reverse mutagenesis primer
5'- CTA GTG GAG CAG TAA CCA GTT CCT TCT TGC CCG -3'
Source
- BAT2 from Saccharomyces cerevisiae (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=1015896&from=472&to=1602&view=gbwithparts Z49648 REGION: 472..1602])
- [http://db.yeastgenome.org/cgi-bin/locus.pl?sgdid=S000003909 BAT2 SGD entry]
References
<biblio>
- Eden-JBC-1996 pmid=8702755
- Kispal-JBC-1996 pmid=8798704
- Eden-ApplMicrobiolBiotechnol-2001 pmid=11341309
- Yoshimoto-ApplMicrobiolBiotechnol-2002 pmid=12172617
- Lilly-2006 pmid=16879424
</biblio>