Coding
BAT2

Part:BBa_J45008:Design

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)

branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1030
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
  • Constructed by PCR

Template

Saccharomyces cerevisiae strain ACLY387 genome

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA Gat gac ctt ggc acc cct aga cg -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAg ttc aaa tca gta aca acc -3'

Forward mutagenesis primer

5'- CGG GCA AGA AGG AAC TGG TTA CTG CTC CAC TAG -3'

Reverse mutagenesis primer

5'- CTA GTG GAG CAG TAA CCA GTT CCT TCT TGC CCG -3'

Source

  • BAT2 from Saccharomyces cerevisiae (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=1015896&from=472&to=1602&view=gbwithparts Z49648 REGION: 472..1602])
  • [http://db.yeastgenome.org/cgi-bin/locus.pl?sgdid=S000003909 BAT2 SGD entry]

References

<biblio>

  1. Eden-JBC-1996 pmid=8702755
  2. Kispal-JBC-1996 pmid=8798704
  3. Eden-ApplMicrobiolBiotechnol-2001 pmid=11341309
  4. Yoshimoto-ApplMicrobiolBiotechnol-2002 pmid=12172617
  5. Lilly-2006 pmid=16879424

</biblio>