Part:BBa_J45400

3-methylbutanal generator

The 3-methylbutanal generator (BBa_J45400) catalyzes the conversion of the cellular metabolite leucine to the banana odor precursor 3-methylbutanal. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45398). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

BBa_J45008 encodes a branched-chain amino acid transaminase derived from the BAT2 gene from Saccharomyces cerevisiae. BAT2 catalyzes the first step in isoamyl alcohol biosynthesis: conversion of leucine to α-ketoisocaproate via enzymatic reaction 2.6.1.42. BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. An alcohol dehydrogenase from the cellular chassis reduces 3-methylbutanal to isoamyl alcohol in an NADH-dependent reaction.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1250
  Illegal EcoRI site found at 2151
  Illegal EcoRI site found at 2850
  Illegal EcoRI site found at 3036
  Illegal SpeI site found at 1944
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1250
  Illegal EcoRI site found at 2151
  Illegal EcoRI site found at 2850
  Illegal EcoRI site found at 3036
  Illegal SpeI site found at 1944
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1250
  Illegal EcoRI site found at 2151
  Illegal EcoRI site found at 2850
  Illegal EcoRI site found at 3036
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1250
  Illegal EcoRI site found at 2151
  Illegal EcoRI site found at 2850
  Illegal EcoRI site found at 3036
  Illegal SpeI site found at 1944
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1250
  Illegal EcoRI site found at 2151
  Illegal EcoRI site found at 2850
  Illegal EcoRI site found at 3036
  Illegal SpeI site found at 1944
  Illegal AgeI site found at 1114
  
• 1000
  COMPATIBLE WITH RFC[1000]