Difference between revisions of "Help:Terminators"

 
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[[Image:Part icon terminator.png]]
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[[Category:Terminators]]
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[[Terminators|< Back to Terminators]]
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Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=terminator terminator parts]!</small>
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<hr>
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A terminator is a transcriptional regulator of the conversion of DNA to RNA.  Its sequence indicates the end of a functional operon (ie. a [[Help:Protein coding|coding region]] attached to a regulatory region)
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{{:Terminators/Overview}}
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*[[Help:Terminators/Mechanism|'''How''']] do terminators work?
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*[[Help:Terminators/Glossary|'''Glossary''']] of terms relating to terminators
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*[[Help:Terminators/Design|'''How to design a terminator''']]
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*[[Help:Terminators/Construction|'''How to construct an terminator''']]
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*[[Help:Terminators/Measurement|'''How termination efficiencies are measured''']]
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*[[Help:Terminators/Further reading|'''Further reading''']] about terminator sequence and function
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*[[Terminators/Catalog|'''Browse''']] the terminator parts available from the registry

Latest revision as of 21:41, 20 July 2017

< Back to Terminators

A rho-independent transcriptional terminator. Image courtesy of Kingsford et al..

Terminators are genetic parts that usually occur at the end of a gene or operon and cause transcription to stop. In prokaryotes, terminators usually fall into two categories (1) rho-independent terminators and (2) rho-dependent terminators.

Rho-independent terminators are generally composed of palindromic sequence that forms a stem loop rich in G-C base pairs followed by several T bases. The conventional model of transcriptional termination is that the stem loop causes RNA polymerase to pause and transcription of the poly-A tail causes the RNA:DNA duplex to unwind and dissociate from RNA polymerase.

All the E. coli terminators in the Registry are rho-independent terminators. Rho-dependent terminators are not included, because rho-dependent terminators are not specified by sequence.