Difference between revisions of "Collections/JUMP"

Revision as of 21:17, 12 July 2022

This collection is under curation by iGEM HQ. New parts and information are currently being added to this page.

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA). The collection includes plasmids with 10 different replication origins allowing for flexibility in both copy number and chassis. A selection of the JUMP collection has been made available to iGEM teams through the iGEM 2022 Distribution.

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Level 1

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428341	pJUMP29-1A	pBBR322 (Medium)	Kanamycin	Level 1	A	BBa_J428326	Kit Plate 1 Well 2A
BBa_J428342	pJUMP29-1B	pBBR322 (Medium)	Kanamycin	Level 1	B	BBa_J428326	Kit Plate 1 Well 2C
BBa_J428343	pJUMP29-1C	pBBR322 (Medium)	Kanamycin	Level 1	C	BBa_J428326	Kit Plate 1 Well 2E
BBa_J428344	pJUMP29-1D	pBBR322 (Medium)	Kanamycin	Level 1	D	BBa_J428326	Kit Plate 1 Well 2G

*More...*
Name	Description	Created by	resistance	origin	type
BBa_J428341	pJUMP29-1A KanR Type IIS Level 1 vector for A modules	Marcos Valenzuela-Ortega	kanamycin	pBBR322	Plasmid_Backbone
BBa_J428342	pJUMP29-1B KanR Type IIS Level 1 vector for B modules	Marcos Valenzuela-Ortega	kanamycin	pBBR322	Plasmid_Backbone
BBa_J428343	pJUMP29-1C KanR Type IIS Level 1 vector for C modules	Marcos Valenzuela-Ortega	kanamycin	pBBR322	Plasmid_Backbone
BBa_J428344	pJUMP29-1D KanR Type IIS Level 1 vector for D modules	Marcos Valenzuela-Ortega	kanamycin	pBBR322	Plasmid_Backbone
BBa_J428345	pJUMP21-1A KanR Type IIS Level 1 vector. Origin R6K (pir conditional).	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428346	pJUMP22x-1A KanR Type IIS Level 1 vector. Origin RK2 (broad-host-range).	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428347	pJUMP23-1A KanR Type IIS Level 1 vector. Origin pBBR1 (medium-copy; broad-host-range)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428348	pJUMP24-1A KanR Type IIS Level 1 vector. Origin pRO1600/ColE1.	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428349	pJUMP25-1A KanR Type IIS Level 1 vector. Origin RSF1010 (Broad-host-range)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428350	pJUMP26-1A KanR Type IIS Level 1 vector. Origin p15A (medium copy number)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428351	pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428352	pJUMP27ts-1A KanR Type IIS Level 1 vector. Thermosensitive origin pSC101 (low copy number)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428353	pJUMP28-1A KanR Type IIS Level 1 vector. Origin pUC (high copy number)	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428355	pJUMP29-1D Prime KanR Type IIS Level 1 vector for D modules	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone
BBa_J428356	pJUMP29-1E KanR Type IIS Level 1 vector for E modules	Marcos Valenzuela-Ortega	kanamycin		Plasmid_Backbone

Loop Compatible

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428381	pOdd-1 (pJUMP26)	p15A (Medium)	Kanamycin	Level 1	1	BBa_J04454	Kit Plate 1 Well 10I
BBa_J428382	pOdd-2 (pJUMP26)	p15A (Medium)	Kanamycin	Level 1	2	BBa_J04454	Kit Plate 1 Well 10K
BBa_J428383	pOdd-3 (pJUMP26)	p15A (Medium)	Kanamycin	Level 1	3	BBa_J04454	Kit Plate 1 Well 10M
BBa_J428384	pOdd-4 (pJUMP26)	p15A (Medium)	Kanamycin	Level 1	4	BBa_J04454	Kit Plate 1 Well 10O

Level 2

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428361	pJUMP49-2A	pBBR322 (Medium)	Spectinomycin	Level 2	A	BBa_J428327	Kit Plate 1 Well 6A
BBa_J428362	pJUMP49-2B	pBBR322 (Medium)	Spectinomycin	Level 2	B	BBa_J428327	Kit Plate 1 Well 6C
BBa_J428363	pJUMP49-2C	pBBR322 (Medium)	Spectinomycin	Level 2	C	BBa_J428327	Kit Plate 1 Well 6E
BBa_J428364	pJUMP49-2D	pBBR322 (Medium)	Spectinomycin	Level 2	D	BBa_J428327	Kit Plate 1 Well 6G

Level 0

Plasmid	Name	Origin (Copy)	ABr	Level	Selection	2022 Distribution Location
BBa_J428388	pJUMP19-min2	pUC (High)	Ampicillin	Level 0	No argument specified with partinfo tag.	Kit Plate 1 Well 10A
BBa_J428387	pJUMP19-Pac	pUC (High)	Ampicillin	Level 0	No argument specified with partinfo tag.	Kit Plate 1 Well 10C
BBa_J428386	pJUMP19-Tac	pUC (High)	Ampicillin	Level 0	No argument specified with partinfo tag.	Kit Plate 1 Well 10E
BBa_J428385	pJUMP18-Uac	pUC (High)	Ampicillin	Level 0	No argument specified with partinfo tag.	Kit Plate 1 Well 10G

Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.

Revision as of 21:13, 12 July 2022 (view source) Vinoo (Talk \| contribs) m ← Older edit		Revision as of 21:17, 12 July 2022 (view source) Vinoo (Talk \| contribs) m Newer edit →
Line 11:		Line 11:
	{{Template:Collection/JUMP/Level1/Plasmids}}		{{Template:Collection/JUMP/Level1/Plasmids}}

−	<parttable>~~collection_jump~~</parttable>	+	<parttable>collection_jump_level1</parttable>