Part:BBa_J428362

pJUMP49-2B SpecR Type IIS Level 2 vector for B modules

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number)

JUMP Collection

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA).

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428361	pJUMP49-2A	pBBR322 (Medium)	Spectinomycin	Level 2	A	BBa_J428327	Kit Plate 1 Well 6A
BBa_J428362	pJUMP49-2B	pBBR322 (Medium)	Spectinomycin	Level 2	B	BBa_J428327	Kit Plate 1 Well 6C
BBa_J428363	pJUMP49-2C	pBBR322 (Medium)	Spectinomycin	Level 2	C	BBa_J428327	Kit Plate 1 Well 6E
BBa_J428364	pJUMP49-2D	pBBR322 (Medium)	Spectinomycin	Level 2	D	BBa_J428327	Kit Plate 1 Well 6G

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 2991
  Illegal suffix found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2991
  Illegal NheI site found at 2
  Illegal SpeI site found at 17
  Illegal PstI site found at 31
  Illegal NotI site found at 24
  Illegal NotI site found at 2997
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2991
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2991
  Illegal suffix found at 17
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2991
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3006
  Illegal SpeI site found at 17
  Illegal PstI site found at 31
  Illegal NgoMIV site found at 1039
  Illegal NgoMIV site found at 1441
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 3013
  Illegal BsaI.rc site found at 10
  Illegal SapI.rc site found at 2239