Plasmid_Backbone

Part:BBa_J428385

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-16)


pJUMP18-Uac Level 0 Universal Acceptor Plasmid

pJUMP18-Uac Level 0 Universal Acceptor Plasmid

High copy number pUC origin.

The fusion sites of the Level 0 part, type generated are introduced during domestication. pJUMP18-Uac is paired with a sfGFP reporter gene.

Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2587
    Illegal suffix found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2587
    Illegal SpeI site found at 19
    Illegal PstI site found at 33
    Illegal NotI site found at 26
    Illegal NotI site found at 2593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2587
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2587
    Illegal suffix found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2587
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2602
    Illegal SpeI site found at 19
    Illegal PstI site found at 33
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 1493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2291


[edit]
Categories
Parameters
None