Part:BBa_J428344

pJUMP29-1D KanR Type IIS Level 1 vector for D modules

OriV 9 (pBBR322/ROP; medium copy number)

JUMP Collection

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA).

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428341	pJUMP29-1A	pBBR322 (Medium)	Kanamycin	Level 1	A	BBa_J428326	Kit Plate 1 Well 2A
BBa_J428342	pJUMP29-1B	pBBR322 (Medium)	Kanamycin	Level 1	B	BBa_J428326	Kit Plate 1 Well 2C
BBa_J428343	pJUMP29-1C	pBBR322 (Medium)	Kanamycin	Level 1	C	BBa_J428326	Kit Plate 1 Well 2E
BBa_J428344	pJUMP29-1D	pBBR322 (Medium)	Kanamycin	Level 1	D	BBa_J428326	Kit Plate 1 Well 2G

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 2944
  Illegal suffix found at 12
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2944
  Illegal SpeI site found at 13
  Illegal PstI site found at 27
  Illegal NotI site found at 20
  Illegal NotI site found at 2950
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2944
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2944
  Illegal suffix found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2944
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2959
  Illegal SpeI site found at 13
  Illegal PstI site found at 27
  Illegal NgoMIV site found at 1386
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 2184