Plasmid_Backbone

Part:BBa_J428386

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-16)


pJUMP19-Tac Level 0 Terminator acceptor vector with J23100 upstream and eGFP downstream

Terminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.

Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3026
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3032
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3026
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3026
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3026
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3041
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 853
    Illegal NgoMIV site found at 982
    Illegal NgoMIV site found at 1476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2274


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Categories
Parameters
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