Difference between revisions of "Chassis"

Latest revision as of 13:11, 21 October 2019

Biologists are familiar with the concept of organisms. The iGEM Registry of Standard Biological Parts in most cases uses the concept of "chassis", "the conceptual extension of a living cell" [1].

		Escherichia coli chassis (?): Most parts in the Registry operate in E. coli.
		Bacillus subtilis chassis (?): Bacillus subtilis is a model gram-positive bacterium.
		Cell-free chassis (?): In vitro transcription/translation systems can be useful for some synthetic biological systems.
		Escherichia coli (?): Most parts in the Registry function in E. coli.
		Yeast (?): Yeast are simple eukaryotes.
		Bacteriophage T7 (?): Bacteriophage T7 is an obligate lytic phage of E. coli.
		Bacillus subtilis (?): Bacillus subtilis is a model gram-positive bacterium.
		Chlamydomonas reinhardtii (?): single-cell fresh water green alga, functioning as plant model organism.
Marchantia Polymorpha		Marchantia Polymorpha (?): Cambridge iGEM 2014 are introducing the first plant chassis to iGEM: Marchantia Polymorpha, a primitive liverwort. They are building a library of biobricks optimized for this Eukaryote. This diploid, small and fast reproducing plant is the ideal chassis for engineering plants. Its genome has recently been fully sequenced and interest in this little plant is rising fast.
Azotobacter vinelandii		Azotobacter vinelandii (?): Azotobacter vinelandii is gram-negative Pseudomonadales bacteria introduced by CUHK IGEM 2014. It provides an intracellular anaerobic environment in a extracellular aerobic environment. Hence, this chassis is suitable for engineering oxygen sensitive protein. Most parts optimized for E. coli may also function in this chassis.
		MammoBlocks (?): MammoBlocks are a new category of BioBrick introduced by the MIT iGEM team in 2010 and continued in 2011. There are now dozens of MammoBlocks suitable for rapid expression in mammalian cells.
		Mesoplasma florum (?): Mesoplasma florum is a particularly simple model organism.
		Magnetotaxis (?): magnetotactic strain AMB-1 with BioBrick-compatible toolkit enables development of synthetic biology into magnetotaxis and beyond.

References:

[1] [http://www.sciencedirect.com/science/article/pii/S0014579311009082 "Scaling up synthetic biology: Do not forget the chassis", FEBS Letters 586 (2012) 2129–2137.]