Difference between revisions of "Add a Part to the Registry"
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The most important feature of a standard biological part should be that a user of the part does not have to talk to the part's designer. This is achieved by completely documenting the part. | The most important feature of a standard biological part should be that a user of the part does not have to talk to the part's designer. This is achieved by completely documenting the part. | ||
| − | Users can [[DNA_Submission|submit samples]] of their parts to the Registry in the BioBrick plasmid ([https://parts.igem.org/wiki/index.php/Part:pSB1C3 pSB1C3]), and flanked by the [[Help:Prefix-Suffix|BioBrick prefix and suffix]]. The prefix and suffix are ''not'' included in the sequence of the part when documented on the Registry. | + | Users can [[DNA_Submission|submit samples]] of their parts to the Registry in the BioBrick plasmid ([https://parts.igem.org/wiki/index.php/Part:pSB1C3 pSB1C3]), and flanked by the [[Help:Prefix-Suffix|BioBrick prefix and suffix]]. The prefix and suffix are ''not'' included in the sequence of the part when documented on the Registry. Sample submission is no longer a requirement for the iGEM competition. |
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Revision as of 20:30, 17 August 2019
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Adding Parts
Members of iGEM teams and labs can add three kinds of parts to the Registry: Basic Parts, Composite Parts, and Construction Intermediates.
Parts must have a DNA sequence. Registry users should document, characterize, and measure their parts, and provide that information on their part pages.
The most important feature of a standard biological part should be that a user of the part does not have to talk to the part's designer. This is achieved by completely documenting the part.
Users can submit samples of their parts to the Registry in the BioBrick plasmid (pSB1C3), and flanked by the BioBrick prefix and suffix. The prefix and suffix are not included in the sequence of the part when documented on the Registry. Sample submission is no longer a requirement for the iGEM competition.
Basic Parts
Basic Parts are discrete functional units of DNA: promoters, ribosome binding sites, protein coding regions, etc. They cannot be subdivided into smaller component parts. DNA for a basic part may be obtained by ''de novo'' synthesis, by primer extension and PCR, or via other techniques. Like all parts submitted to the Registry, a Basic Part sample is stored in a plasmid (pSB1C3), and flanked by the BioBrick prefix and suffix. The prefix and suffix are ''not'' included in the sequence of the part when documented on the Registry. BBa_K863006 is a good example of a well documented Basic Part. Read our help page on adding a basic part.
Composite Parts
Composite Parts are functional units made from an ordered series of basic parts or other composite parts. '''Explicit base pairs of DNA cannot be entered in as sequence for these parts''' (parts which do require you to manually enter sequence are Basic Parts). The Registry's software provides information and sequence for all the basic parts that you list as components of your composite part. While the Registry provides the sequence from the component parts specified, the function and design issues of the composite part should be documented in detail. BBa_K1150020 is a good example of a well documented Composite Part. Read our help page on adding a composite part.
Construction Intermediates
Construction Intermediates have no specific function and are just the result of assembling two parts together. They require no further documentation. Often they are unwanted byproducts of construction They all have the type 'Intermediate' and part names of the form 'BBa_Snnnnn'. These part names are automatically assigned by the Registry software. Once you enter your intermediate part in the Registry, you will be able to use BioBrick Blast to check your assembly's sequence and your part will show up in the subpart and superpart search functions. If you send us the DNA, we will be able to share your work with others and include it in assemblies done by the Registry.
Useful Help Pages
Add a Part to the Registry: Related Registry Help Pages
- Add a Basic Part - A tutorial on how to add a basic part
- Add a Composite Part - A tutorial on how to add a composite part
- Scars - Information on assembly scars, and how to specify them for a composite part
- Synthesis - Things to keep in mind if you're adding and documenting a part you've synthesized
- Assembly Compatibility - Make sure your part is compatible with an assembly standard
- Twins - Parts are twins if they have the same sequence
- Document Parts - Recommendations on how to document your parts
- Make a Contribution - Improve existing parts
Have questions on adding a part to the Registry? Send an email to hq (at) igem . org.
Deleting A Part
You can delete a part by going to a part's "Hard Information" and setting the DNA status to "deleted".
FAQ
What's the difference between a basic and composite part?
- Basic Parts are discrete functional units of DNA. They cannot be subdivided into smaller component parts.
- Composite Parts are functional units made from an ordered series of basic parts or other composite parts.
In order to create a composite part, each basic part that composes it must already be added and documented to the Registry.
Can I add RNA parts or Proteins (AA)?
No the Registry only accepts DNA. For RNA, you would use the DNA sequence that transcribes your RNA sequence, and give it a part type of RNA.
Tip: Avoid special characters when adding a part
Special characters like ' / - _ in the short description may cause issues with generating the part pages.
How do I delete a part?
You can delete a part by going to a part's "Information" page and setting the DNA status to "deleted".
I synthesized my entire device. Can I add it as a basic part and give it the correct part type?
No, this should be added as a composite part. You should make sure that the basic parts that compose these parts are on the Registry (already exist or you'll be adding them as new basic parts if necessary). You can can go to part tools > edit sequence and features and generate your composite part without BioBrick scars (Blunt Assembly). You can create a small basic part(s) with a part type of "Scar," and enter those in between the basic parts that constitute your composite part.
I want to add a part but do not know the sequence
All users should know their part's sequence before working with it. However, you can still add and document your part and add/edit the sequence later by going to part tools >> edit sequence and features.
How do I document an improvement on an existing part on the Registry?
There are many different ways to improve an existing part, from adding measurement data to codon-optimization. Please see our help page on contributions. Note that the following page is on how to document different types of contributions or improvements. Make sure to read through the medal criteria for a given iGEM year to understand what the judges are looking for.
How do I see if my part is compatible?
The Sequence & Features box will show you assembly compatibility.
The Assembly Compatibility checks that a part will meet the requirements for the Registry's supported assembly standards. It will show if there are any illegal restriction sites for a part. The above image shows a basic part the is compatible with RFC 10 and 1000, amongst others.
Note: All parts submitted to the Registry for competition evaluation (medal criteria, awards) must be BioBrick (RFC[10] compatible or Type IIS RFC[1000], unless an exemption has been given.
See the competition delivearbles page for part requirements
We have a part that we'd like to add, but it is not BioBrick RFC10 compatible or iGEM Type IIS compatible
Yes, please add and document it on the Registry. But make sure to see the parts deliverables page for competition requirements.
Should I include the BioBrick prefix and suffix or iGEM Type IIS prefix and suffix in my part's sequence?
No, your part's sequence should not include the prefix and suffix. While your parts should be assembly compatible, the prefix and suffix are an element of the plasmid backbone, not the part.
How do I upload images to part pages
Use uploads.igem.org to access your upload space. You can then use html tags in the wiki code:
< html ><img src = "url goes here">< /html > (removing the spaces in the html tags)
I have made a part, but cannot edit a wiki page
Make sure to follow the login instructions here, as you need to login to igem.org, and then into the part wiki.