Difference between revisions of "Help:Terminators"
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[[Image:Part icon terminator.png]]
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[[Category:Terminators]]
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<small>
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[[Terminators|< Back to Terminators]]
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Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=terminator terminator parts]!</small>
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<hr>
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A terminator (short for "transcriptional terminator") is a stretch of DNA which halts the process of transcription (making the RNA copy of a DNA sequence). 
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{{:Terminators/Overview}}
  
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*[[Help:Terminators/Mechanism|'''How''']] do terminators work?
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==Stem-loop type terminators==
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*[[Help:Terminators/Glossary|'''Glossary''']] of terms relating to terminators
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[[Image:B0011 - stem loop terminator.png|thumb|An example of a stem-loop coding region (location indicated by the hairpin picture) in [[Part:BBa_B0011]]]]
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*[[Help:Terminators/Design|'''How to design a terminator''']]
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In our prokaryotic BioBrick [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell host cells] these [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=terminator terminator parts] are often palindromic (same sequence backwards and forwards on the two DNA strands) and the RNA transcript (the second half of which is the reverse complement of the first half) forms a stem-loop structure by folding back on itself. This structure then terminates transcription.<br>
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*[[Help:Terminators/Construction|'''How to construct an terminator''']]
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One example of a BioBrick which uses this method is the terminator [[Part:BBa_B0011]], which has the sequence"<font color = "green">aaaagccagattat</font><font color = "red">taatccggctttt</font>" on one DNA strand.  The corresponding RNA transcript (with U's in place of T's) can fold back into a hairpin with the green nucleotides running right-to-left and the red left-to-right.  (Try drawing out the base-paired structure -- the middle of the hairpin has three unpaired bases and the stem has one mismatch.)
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*[[Help:Terminators/Measurement|'''How termination efficiencies are measured''']]
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*[[Help:Terminators/Further reading|'''Further reading''']] about terminator sequence and function
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==Poly-A tails==
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*[[Terminators/Catalog|'''Browse''']] the terminator parts available from the registry
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In eukaryotic hosts such as [https://parts.igem.org/wiki/index.php/Yeast yeast], a string of adenosine ("A") nucleotides is the primary method through which termination of transcription occurs.  This is mediated by exonucleases (enzymes which cut at this recognition sequence).  A popular "poly-A" motif is "<b>AAUAAA</b>".
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==Rho type terminators==
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Another method which cells use to terminate a sequence is through the action of the Rho protein.
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Latest revision as of 21:41, 20 July 2017

< Back to Terminators

A rho-independent transcriptional terminator. Image courtesy of Kingsford et al..

Terminators are genetic parts that usually occur at the end of a gene or operon and cause transcription to stop. In prokaryotes, terminators usually fall into two categories (1) rho-independent terminators and (2) rho-dependent terminators.

Rho-independent terminators are generally composed of palindromic sequence that forms a stem loop rich in G-C base pairs followed by several T bases. The conventional model of transcriptional termination is that the stem loop causes RNA polymerase to pause and transcription of the poly-A tail causes the RNA:DNA duplex to unwind and dissociate from RNA polymerase.

All the E. coli terminators in the Registry are rho-independent terminators. Rho-dependent terminators are not included, because rho-dependent terminators are not specified by sequence.