Difference between revisions of "Part:BBa F2620"
| Line 11: | Line 11: | ||
|width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous | |width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous | ||
|width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | |width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | ||
| − | |width='210px' align='left'| | + | |width='210px' align='left'|9.5 mins (50% of max. output) |
|- | |- | ||
|[[Output Swing|''Output Swing:'']] | |[[Output Swing|''Output Swing:'']] | ||
Revision as of 23:34, 5 October 2006
|
|
|
Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
| Characteristics | |||
| Input Swing: | 0.1 - 1000 nM 3OC6HSL, exogenous | L/H Response time: | 9.5 mins (50% of max. output) |
| Output Swing: | 21±3 - 590±9 GFP molecules cfu-1 s-1 | Genetic Stability: | >92/74 generations (Low/High Input) |
| Switch Point: | 1E-8 M 3OC6HSL, exogenous | Performance Stability: | >92/74 generations (Low/High Input) |
| Transfer Function |
Specificity |
| Response time |
Stability |
| Demand | |||
| Translation Demand: (Low/High Input) |
5040/141600 charged tRNA cfu-1 s-1 336/9440 ribosomes sequestered cfu-1 |
Growth Rate: | 54/59 min doubling time (Low/High Input) |
Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.