Difference between revisions of "Part:BBa F2620"
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|width='340px' align="left"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br> | |width='340px' align="left"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br> | ||
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|align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br> | |align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br> | ||
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|align="left"|[[Part:BBa_F2620:Stability|'''Stability''']]<br><center> | |align="left"|[[Part:BBa_F2620:Stability|'''Stability''']]<br><center> | ||
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Revision as of 18:07, 5 October 2006
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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
| Characteristics | |||
| Input Swing: | 0.1 - 1000 nM 3OC6HSL, exogenous | L/H Response time: | 25 mins |
| Output Swing: | 13.6 - 490.2 GFP molecules/s.cfu | Genetic Stability: | >92/74 generations (Low/High Input) |
| Switch Point: | 8 nM 3OC6HSL, exogenous | Performance Stability: | >92/74 generations (Low/High Input) |
| Transfer Function |
Specificity |
| Response time |
Stability |
| Demand | |||
| Transcription Demand: | ## NTP per second ## polymerases sequestered |
Translation Demand: | # charged tRNA per second # ribosomes sequestered |
Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing BBa_C0040.