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- Zhang Lab devised the SHERLOCK technique which uses isothermal preamplification w Zhang et al. have further devised SHERLOCKv2 [2] which is an advancement of the S19 KB (2,810 words) - 20:42, 21 October 2021
- ...if the purified sortases connect two proteins that carry the important Sortase-recognition <li>(M)GGGG-mCherry, a protein sample that might still carry an N-terminal106 KB (11,833 words) - 14:02, 19 October 2021
- <b>Author: Aaron Zhexuan Zhang<br> To demonstrate the auto-secretion of sfGFP described by Zhang et al.<sup>2</sup> we designed two constructs, each containing the Npu DnE52 KB (7,992 words) - 07:51, 8 October 2022
- ...ome P450 enzymes. CPR provides the necessary electrons for P450 enzymes to carry out the oxidation reactions. This electron transfer is crucial for enabling ..."https://doi.org/10.1021/acs.jafc.2c00754">Zhang, J., Wang, X., Zhang, X., Zhang, Y., Wang, F., & Li, X. (2022).Sesquiterpene Synthase Engineering and T4 KB (553 words) - 03:19, 2 October 2024
- <p>To validate the ability of EXOtic to carry GPC3 mRNA in exosomes, we employed qPCR to measure the GPC3 mRNA content <p id="r2">[2] Zhang, M., Hu, S., Liu, L., Dang, P., Liu, Y., Sun, Z., Qiao, B., & Wang, C. (2024 KB (592 words) - 11:57, 2 October 2024
- pEcCas plasmid carry the cas 9 protein which can cut the target gene. <p> Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the p2 KB (363 words) - 15:00, 12 October 2023
- [6] Guo, Y., Qin, X., Tang, Y., Ma, Q., Zhang, J., & Zhao, L. (2020). Cota Laccase, a novel aflatoxin oxidase from ba [7] Yang, P., Xiao, W., Lu, S., Jiang, S., Zheng, Z., Zhang, D., Zhang, M., Jiang, S., & Jiang, S. (2021). Recombinant expression of trametes40 KB (6,051 words) - 10:07, 12 October 2023
- ...tion, which represents the success of construction. This meant that we can carry out subsequent cell transfection and characterization qualitative detection 2. Wang, Z., Shi, X., Zhang, H., Yu, L., Cheng, Y., Zhang, H., & Duan, W. (2017). Discovery of cycloalkyl-fused N-thiazol-2-yl-benzam10 KB (1,466 words) - 14:53, 12 October 2022
- ...tion, which represents the success of construction. This meant that we can carry out subsequent cell transfection and characterization qualitative detection 2. Wang, Z., Shi, X., Zhang, H., Yu, L., Cheng, Y., Zhang, H., & Duan, W. (2017). Discovery of cycloalkyl-fused N-thiazol-2-yl-benzam9 KB (1,432 words) - 08:29, 26 September 2022
- ...spin column(Merck, USA). Noticeably, even the <b>HRT2-SP protein does not carry any His-tags and is smaller than threshold (<30kDa) of the spin column filt 2. Yang J, Zhang L, Zhang C, Lu Y. Exploration on the expression and assembly of virus-like particles17 KB (2,649 words) - 15:43, 29 September 2024
- ...rotocol, so we studied the following articles: Zong, Y et al (2017)[1] and Zhang, H. M. et al(2015). </p> <p> [1]Zong, Y., Zhang, H. M., Lyu, C., Ji, X., Hou, J., Guo, X., ... & Lou, C. (2017). Insulated27 KB (4,267 words) - 13:39, 1 October 2024
- ...his is a method used to determine the concentration of a target DNA[6]. To carry out qPCR, the mRNA from the larvae was first extracted and reverse-transcri [3] Wu, J., Wei, L., He, J., Fu, K., Li, X., Jia, L., Wang, R., & Zhang, W. (2021). Characterization of a novel Bacillus thuringiensis toxin active19 KB (3,052 words) - 13:07, 2 October 2024
- [2]Pan Y, Zhang Y, Jia T, Zhang K, Li J, Wang L. Development of a microRNA delivery system based on bacteri [1]Qi L, Zhang Z, Wang M, Ke Z, Mao H, Deng G, Wang J. One-plasmid double-expression syste16 KB (2,408 words) - 13:26, 2 October 2024
- ...lter spin column (Merck, USA). Importantly, the <b>HRT2-SP protein did not carry any His-tags and thus if non encapsulated, was smaller than the threshold ( 2. Yang J, Zhang L, Zhang C, Lu Y. Exploration on the expression and assembly of virus-like particles18 KB (2,900 words) - 13:34, 2 October 2024
- ...will undergo genetic recombination within bacteria, and their genome will carry the relE gene, thus creating a modified phage. However, the toxin protein i [4]Li GY, Zhang Y, Inouye M, Ikura M. Inhibitory mechanism of Escherichia coli RelE-RelB to4 KB (675 words) - 10:22, 4 July 2020
- Cyanobacteria, as well as algae and higher plants, carry out oxygenic photosynthesis, which requires multiprotein complexes that dri Wang, B., Wang J., Zhang, W. & Meldrum, D. R. (2012) Application of Synthetic Biology in cyanobacter16 KB (2,320 words) - 12:26, 20 October 2021
- In order to carry out a correct characterization of the protein and to be able to use it to m 2: Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. ”BglBrick vector28 KB (4,308 words) - 19:28, 21 October 2021
- ...ant viral vectors. However, conventional viral vectors usually are able to carry around 2kb. The SpCas9 is too large. Our <b>solution</b> is to divide the C Li J, Norville J, Aach J, McCormack M, Zhang D, Bush J et al. Multiplex and homologous recombination–mediated genome e7 KB (1,208 words) - 18:56, 21 October 2016
- ...ant viral vectors. However, conventional viral vectors usually are able to carry around 2kb. The SpCas9 is too large. Our <b>solution</b> is to divide the C Li J, Norville J, Aach J, McCormack M, Zhang D, Bush J et al. Multiplex and homologous recombination–mediated genome e8 KB (1,219 words) - 18:57, 21 October 2016
- ...ositive for the progesterone nanobody insert. Colonies 1, 5, 10, 14 and 15 carry the religated vector without an insert. The gel (1,2% agarose with 1x TAE) [29] E. O. McCullum, B. A. R. Williams, J. Zhang, und J. C. Chaput, „Random mutagenesis by error-prone PCR“, Methods Mol56 KB (8,191 words) - 03:28, 28 October 2020