Designed by: Yiming Dong Group: iGEM24_HS-Alpha (2024-10-01)
CPR
Cytochrome P450 Reductase (CPR) is a coding gene in the S. cerevisiae,which is essential for the activity of cytochrome P450 enzymes. CPR provides the necessary electrons for P450 enzymes to carry out the oxidation reactions. This electron transfer is crucial for enabling the P450-catalyzed hydroxylation of sesquiterpenes, facilitating the conversion of santalene and bergamotene into santalols and bergamotol. More experimental results can be found on the webpage BBa_K5281017.
Verifying the expression of CYP/CPR in S. Cerevisiae
Figure 1. CYP/CPR homologous recombination verification with Colony PCR and Gel Electrophoresis
We also verified the expression of CYP736A167 and SaCPR2 with SDS-PAGE. The kDa of the two enzymes are listed below:
CYP- 55.91 kDa
CPR- 77.65 kDa
Figure 2. CYP/CPR Enzyme Expression verification with SDS PAGE