Protein domains/Cleavage
Cleavage sites are specific peptide sequences, or more often, peptide motifs at which site-specific proteases with cleave or cut the protein. Cleavage sites can be used, for example, to cleave off an affinity tag thereby restoring the natural protein sequence or to inactivate a protein.
| Name | Description | Protease | AA sequence | Length |
|---|---|---|---|---|
| BBa_I712015 | HIV-1 protease cleavage site | 42 | ||
| BBa_I712016 | myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site | 57 | ||
| BBa_J18918 | TEV cleavage site (E. coli) | 21 | ||
| BBa_J18919 | preScission protease cleavage site (E. coli-optimized) | 24 | ||
| BBa_K128002 | TEV tobacco etch virus protease cleavage site | 21 | ||
| BBa_K1680012 | Cleavage site for HCV protease | 36 | ||
| BBa_K1680013 | NDegron | 165 | ||
| BBa_K1947002 | Intein | 603 | ||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | ||
| BBa_K2812003 | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | 776 | ||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | ||
| BBa_K2812005 | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | 1496 | ||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | ||
| BBa_K2812007 | Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter | 2288 | ||
| BBa_K4190006 | Enterokinase Cut Site | 15 | ||
| BBa_K4803013 | 3xPPVCS (PPV protease cleavage site) | 81 | ||
| BBa_K648008 | TEV protease cleaveage site with Standard 25 Prefix/Suffix | 21 | ||
| BBa_K648009 | RecA Cleavage Site with Standard 25 Prefix/Suffix | 21 | ||
| BBa_K772001 | EpCAM Binding Dock: C-terminus | 1159 | ||
| BBa_K772002 | EpCAM Binding Dock: N terminus | 1138 | ||
| BBa_K772006 | GFP - LacZ anchor-inducer protein complex | 1207 | ||
| BBa_K772007 | GFP - LasR anchor-inducer protein complex | 1720 | ||
| BBa_K772008 | GFP - LacZ protein complex with flag tags | 1232 | ||
| BBa_K772009 | GFP - LasR protein complex with flag tags | 1745 | ||
| BBa_K772100 | EpCAM Detection Device | 2305 | ||
| BBa_K772103 | GFP - LacZ protein complex generator | 1269 |
References
- [http://www.expasy.ch/tools/peptidecutter/ Tool for predicting cleavage sites in proteins]