Protein_Domain

Part:BBa_K4803013

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


3xPPVCS (PPV protease cleavage site)

Usage and Biology

PPVCS (Plum pox virus protease cleavage site) is a recognition sequence for PPVp (Plum pox virus protease). This part was stacked in triplicate to more reliably cause cleavage by PPVp. This sequence was designed based on the paper in [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Vlahos, A. E., Kang, J., Aldrete, C. A., Zhu, R., Chong, L. S., Elowitz, M. B., & Gao, X. J. (2022). Protease-controlled secretion and display of intercellular signals. Nature communications, 13(1), 912.



[edit]
Categories
//chassis/eukaryote/human
//proteindomain/cleavage
Parameters
None