Protein_Domain
Intein

Part:BBa_K1947002

Designed by: zhang xucheng   Group: iGEM16_NEFU_China   (2016-10-13)


Intein

This sequence encodes a short polypeptide which can be specifically recognized and spliced by DL-Dithiothreitol（DTT）. In our case we add this short sequence between the recombinant protein which we want to purify and the linker protein Spytag, in order to separate the recombinant protein form the Magnetosome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 437



References

Telenti A, Southworth M, Alcaide F et al(1997) The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal. J Bacteriol 179:6378–6382

[edit]
Categories
//proteindomain/cleavage
Parameters
None