Part:BBa_K323135:Design
VioA and VioB enzymes fused with zinc fingers under pBAD promoter
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1204
Illegal NheI site found at 2568 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 6132 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5657
Illegal AgeI site found at 979
Illegal AgeI site found at 5853 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Part:BBa_K323134 was ligated into a vector containing Part:BBa_K323085 as a back insert. The resulting fragment was then ligated into the BsaI cloning site of Part:BBa_K323110.
Source
The part was designed from three parts (Part:BBa_K323110, Part:BBa_K323085 and Part:BBa_K323134) that our team previously constructed.
References
Balibar CJ, Walsh CT. 2006. In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum. Biochemistry, 45:15444-57
Duran N, Justo GZ, Ferreira CV, Melo PS, Cordi L, Martins D. 2007. Violacein: Properties and biological activities. Biotechnology and Applied Biochemistry, 48: 127-133
Jiang P, Wang H., Zhang C, Lou K, Xing XH. 2010. Reconstruction of the violacein bioynthetic pathway from Duganella sp. B2 in different heterologous hosts. Applied Microbiology and Biotechnology, 86: 1077-1088
Christy B, Nathans D. 1989. DNA binding site of the growth factor-inducible protein Zif268. Proceedings of the National Academy of Sciences, 22: 8737–41
Kinzler KW, Vogelstein B. 1990. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Molecular and Cell Biology, 2: 634–42
Reynolds L, Ullman C, Moore M, Isalan M, West MJ, Clapham P, Klug A, Choo Y. 2003. Repression of the HIV-1 5LTR promoter and inhibition of HIV-1 replication by using engineered zinc-finger transcription factors. Proceedings of the National Academy of Sciences, 4:1615–1620
Ooi AT, Stains CI, Ghosh I, Segal DJ. Sequence enabled Reassembly of beta-lactamase (SEER-LAC) - A Sensitive Method for the Detection of Double-Stranded DNA.
Libri V, Onori A, Fanciulli M, Passananti C, Corbi N. 2004. The artificial zinc finger protein "Blues" binds the enhancer of the fibroblast growth factoer 4 and represses transcription. FEBS Letters, 560: 75-80