Coding
Zif-vioB

Part:BBa_K323134

Designed by: Tina Lebar, Tjaša Stošicki   Group: iGEM10_Slovenia   (2010-10-20)

Zif268_vioB

This part is composited from the Zif268 zinc finger gene (Part:BBa_K323105) and the vioB gene, which codes for the second enzyme in the violacein biosynthetic pathway - a hemoprotein oxidase, which converts the product of VioA to a compound with an unknown chemical formula. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioB enzyme, which binds to a specific DNA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2834
    Illegal AgeI site found at 3030
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None