Collections/JUMP
This collection is under curation by iGEM HQ. New parts and information are currently being added to this page.
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Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA). The collection includes plasmids with 10 different replication origins allowing for flexibility in both copy number and chassis. A selection of the JUMP collection has been made available to iGEM teams through the iGEM 2022 Distribution.
This collection was kindly provided under the OpenMTA by Marcos Valenzuela-Ortega at the French Lab, University of Edinburgh.
For more information please see:
Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003
Level 1
| Plasmid | Name | Origin (Copy) | ABr | Level | Module | Selection | 2022 Distribution Location |
|---|---|---|---|---|---|---|---|
| BBa_J428341 | pJUMP29-1A | pBBR322 (Medium) | Kanamycin | Level 1 | A | BBa_J428326 | Kit Plate 1 Well 2A |
| BBa_J428342 | pJUMP29-1B | pBBR322 (Medium) | Kanamycin | Level 1 | B | BBa_J428326 | Kit Plate 1 Well 2C |
| BBa_J428343 | pJUMP29-1C | pBBR322 (Medium) | Kanamycin | Level 1 | C | BBa_J428326 | Kit Plate 1 Well 2E |
| BBa_J428344 | pJUMP29-1D | pBBR322 (Medium) | Kanamycin | Level 1 | D | BBa_J428326 | Kit Plate 1 Well 2G |
| Name | Description | Created by | resistance | origin | type |
|---|---|---|---|---|---|
| BBa_J428341 | pJUMP29-1A KanR Type IIS Level 1 vector for A modules | Marcos Valenzuela-Ortega | kanamycin | pBBR322 | Plasmid_Backbone |
| BBa_J428342 | pJUMP29-1B KanR Type IIS Level 1 vector for B modules | Marcos Valenzuela-Ortega | kanamycin | pBBR322 | Plasmid_Backbone |
| BBa_J428343 | pJUMP29-1C KanR Type IIS Level 1 vector for C modules | Marcos Valenzuela-Ortega | kanamycin | pBBR322 | Plasmid_Backbone |
| BBa_J428344 | pJUMP29-1D KanR Type IIS Level 1 vector for D modules | Marcos Valenzuela-Ortega | kanamycin | pBBR322 | Plasmid_Backbone |
| BBa_J428345 | pJUMP21-1A KanR Type IIS Level 1 vector. Origin R6K (pir conditional). | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428346 | pJUMP22x-1A KanR Type IIS Level 1 vector. Origin RK2 (broad-host-range). | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428347 | pJUMP23-1A KanR Type IIS Level 1 vector. Origin pBBR1 (medium-copy; broad-host-range) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428348 | pJUMP24-1A KanR Type IIS Level 1 vector. Origin pRO1600/ColE1. | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428349 | pJUMP25-1A KanR Type IIS Level 1 vector. Origin RSF1010 (Broad-host-range) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428350 | pJUMP26-1A KanR Type IIS Level 1 vector. Origin p15A (medium copy number) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428351 | pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428352 | pJUMP27ts-1A KanR Type IIS Level 1 vector. Thermosensitive origin pSC101 (low copy number) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428353 | pJUMP28-1A KanR Type IIS Level 1 vector. Origin pUC (high copy number) | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428355 | pJUMP29-1D Prime KanR Type IIS Level 1 vector for D modules | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone | |
| BBa_J428356 | pJUMP29-1E KanR Type IIS Level 1 vector for E modules | Marcos Valenzuela-Ortega | kanamycin | Plasmid_Backbone |
Loop Compatible
| Plasmid | Name | Origin (Copy) | ABr | Level | Module | Selection | 2022 Distribution Location |
|---|---|---|---|---|---|---|---|
| BBa_J428381 | pOdd-1 (pJUMP26) | p15A (Medium) | Kanamycin | Level 1 | 1 | BBa_J04454 | Kit Plate 1 Well 10I |
| BBa_J428382 | pOdd-2 (pJUMP26) | p15A (Medium) | Kanamycin | Level 1 | 2 | BBa_J04454 | Kit Plate 1 Well 10K |
| BBa_J428383 | pOdd-3 (pJUMP26) | p15A (Medium) | Kanamycin | Level 1 | 3 | BBa_J04454 | Kit Plate 1 Well 10M |
| BBa_J428384 | pOdd-4 (pJUMP26) | p15A (Medium) | Kanamycin | Level 1 | 4 | BBa_J04454 | Kit Plate 1 Well 10O |
Level 2
| Plasmid | Name | Origin (Copy) | ABr | Level | Module | Selection | 2022 Distribution Location |
|---|---|---|---|---|---|---|---|
| BBa_J428361 | pJUMP49-2A | pBBR322 (Medium) | Spectinomycin | Level 2 | A | BBa_J428327 | Kit Plate 1 Well 6A |
| BBa_J428362 | pJUMP49-2B | pBBR322 (Medium) | Spectinomycin | Level 2 | B | BBa_J428327 | Kit Plate 1 Well 6C |
| BBa_J428363 | pJUMP49-2C | pBBR322 (Medium) | Spectinomycin | Level 2 | C | BBa_J428327 | Kit Plate 1 Well 6E |
| BBa_J428364 | pJUMP49-2D | pBBR322 (Medium) | Spectinomycin | Level 2 | D | BBa_J428327 | Kit Plate 1 Well 6G |
| Name | Description | Created by | resistance | origin | type |
|---|---|---|---|---|---|
| BBa_J428361 | pJUMP49-2A SpecR Type IIS Level 2 vector for A modules | Marcos Valenzuela-Ortega | Plasmid_Backbone | ||
| BBa_J428362 | pJUMP49-2B SpecR Type IIS Level 2 vector for B modules | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428363 | pJUMP49-2C SpecR Type IIS Level 2 vector for C modules | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428364 | pJUMP49-2D SpecR Type IIS Level 2 vector for D modules | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428365 | pJUMP41-2A SpecR Type IIS Level 2 vector. Origin R6K (pir conditional). | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428366 | pJUMP42x-2A SpecR Type IIS Level 2 vector. Origin RK2 (broad-host-range). | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428367 | pJUMP43-2A SpecR Type IIS Level 2 vector. Origin pBBR1 (medium-copy, broad-host-range) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428368 | pJUMP44-2A SpecR Type IIS Level 2 vector. Origin pRO1600/ColE1 | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428369 | pJUMP45-2A SpecR Type IIS Level 2 vector. Origin RSF1010 (Broad-host-range) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428370 | pJUMP46-2A SpecR Type IIS Level 2 vector. Origin p15A (medium copy number) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428371 | pJUMP47-2A SpecR Type IIS Level 2 vector. Origin pSC101 (low copy number) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428372 | pJUMP47ts-2A SpecR Type IIS Level 2 vector. Thermosensitive origin pSC101 (low copy number) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428373 | pJUMP48-2A SpecR Type IIS Level 2 vector. Origin pUC (high copy number) | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428374 | pJUMP49-2D' SpecR Type IIS Level 2 vector for D modules | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone | |
| BBa_J428375 | pJUMP49-2E SpecR Type IIS Level 2 vector for E modules | Marcos Valenzuela-Ortega | Spectinomycin | Plasmid_Backbone |
Level 0
| Plasmid | Name | Origin (Copy) | ABr | Level | Selection | 2022 Distribution Location |
|---|---|---|---|---|---|---|
| BBa_J428388 | pJUMP19-min2 | pUC (High) | Ampicillin | Level 0 | No argument specified with partinfo tag. | Kit Plate 1 Well 10A |
| BBa_J428387 | pJUMP19-Pac | pUC (High) | Ampicillin | Level 0 | No argument specified with partinfo tag. | Kit Plate 1 Well 10C |
| BBa_J428386 | pJUMP19-Tac | pUC (High) | Ampicillin | Level 0 | No argument specified with partinfo tag. | Kit Plate 1 Well 10E |
| BBa_J428385 | pJUMP18-Uac | pUC (High) | Ampicillin | Level 0 | No argument specified with partinfo tag. | Kit Plate 1 Well 10G |