Part:BBa_K3657031

MS2 Coat Protein coupled with C-terminal GFP

This is a fusion protein containing MS2 Coat Protein (BBa_K3218006) and C-terminal Split-GFP (BBa_K3657028).

Usage and Biology

MS2 Coat Protein binds specifically the MS2 RNA hairpin (BBa_K3113006).

C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (BBa_K3657027).

This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the N-terminal of the Split-GFP planned for the experiment: BBa_K3657030. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]