Project

Part:BBa_K3218006

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-16)


MS2-N55K, a mutant of the bacteriophage MS2 coat protein with enhanced affinity

The bacteriophage MS2 coat protein which is a N55K mutant. This mutation increases the affinity for the MS2 hairpin loop to which the MS2 protein binds. This part was used as part of the guidance system for the mutation mechanism. The MS2 protein can be fused to the AID (BBa_K103001) and then be used to selecet regions of DNA to target.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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