Intermediate

Part:BBa_K539626

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Taiwan   (2011-09-27)

a RBS (B0034)+ilvD

Built in strong expressing RBS (Part:BBa_B0034) and ilvD (Part:BBa_K539712).The sequence of ilvD had been mutated to delet the function of RE site at the origin in order to apply to the iGEM part.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1525
    Illegal BsaI site found at 1782


[edit]
Categories
Parameters
n/aa RBS (B0034)+ilvD