Coding
Part:BBa_K539712
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-20)
ilvD (dihydroxyacid dehydratase)
This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway.
Following is the isobutanol biosythesis pathway.
Our team has mutated the function of RE site within the original ilvD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1721
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1507
Illegal BsaI site found at 1764
[edit]
Categories
Parameters
None |