Part:BBa_K4390041
arsR regulated mCherry expression
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This is a level 1 part formed by assembly of the following level 0 parts:
| Promoter | K190015 |
| RBS | B0034 |
| N-O-C part | K180008 |
| Terminator | K4390001 |
Usage and Biology
mCherry is a red fluorescent protein which derived from DsRed of Discosoma (Shaner, N. C. et al., 2004). This protein will generate bright red colour when expressed which is always been used as reporter in research. ArsR is a repressor for arsenical resistance operons in bacteria. In gram-negative bacteria, arsR can bind to the promoter of arsenical resistance operons which repress the expression. This repression activity is controlled by arsenic apperance. When arsenic is appeared in the environment, the arsR will bind to arsenic ions which will no longer be able to bind to the promoter and release the arsenical resistance operons to be expressed (Wu, J. and Rosen, B. P., 1991; Diorio, C. et al., 1995).
In this designed part, mCherry will be expressed under regulation of arsR, thus mCherry will only be expressed when there are arsenic present in the environment. In iGEM22_Edinburgh-UHAS_Ghana SELIS design, the expressed mCherry will be used to detect the present of heavy metals, the expression of mCherry will perform a red colour when arsenic is present. For further information please refer to SELIS arsR evolution construct.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 448
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 448
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 448
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 448
- 1000COMPATIBLE WITH RFC[1000]
References
D'Oelsnitz, S. et al., (2022) Using fungible biosensors to evolve improved alkaloid biosyntheses. Nature chemical biology. 18 (9), 981–989.
Johnson, A. D. et al. (1979) Interactions between DNA-Bound Repressors Govern Regulation by the $\lambda $ Phage Repressor. Proceedings of the National Academy of Sciences - PNAS. 76 (10), 5061–5065.
Shaner, N. C. et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology. 22 (12), 1567–1572.
| None |