Part:BBa_K4390040
merR controlled lambda cI expression
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This is a level 1 part formed by assembly of the following level 0 parts:
| Promoter | K346002 |
| RBS | B0034 |
| N-O-C part | K4390037 |
| Terminator | K4390001 |
Usage and Biology
MerR is a repressor for mercury resistance operons in bacteria. In gram-negative bacteria, the dimeric merR can bind to the promoter of mercury resistance operons which repress the expression. This repression activity is controlled by mercury apperance. When mercury is appeared in the environment, the merR protein will undergo conformational change which will no longer be able to bind to the promoter and release the mercury resistance operons to be expressed (Lund, P. A. & Brown, N. L., 1989). Lambda cI is a transcriptional repressor which allows Lambda phage to establish and maintain latency after infect E. coli. It regulates the entry of lytic cycle by repressing the lytic promoters (Johnson, A. D. et al., 1979). This Lambda cI sequence was codon optimised for expression in E. coli K12, and was be used in Seamless Enrichment of Ligand-Inducible Sensors (SELIS) as the repressor (d’Oelsnitz, S. et al., 2022).
In this designed part, Lambda cI will be expressed under regulation of merR, thus Lambda cI will only be expressed when there are mercury present in the environment. In iGEM22_Edinburgh-UHAS_Ghana SELIS design, the expressed Lambda cI will be used to repress CmR gene which generates chloramphenicol resistance in bacteria. For further information please refer to any of SELIS merR evolution construct and SELIS mutated merR evolution construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
D'Oelsnitz, S. et al., (2022) Using fungible biosensors to evolve improved alkaloid biosyntheses. Nature chemical biology. 18 (9), 981–989.
Johnson, A. D. et al. (1979) Interactions between DNA-Bound Repressors Govern Regulation by the $\lambda $ Phage Repressor. Proceedings of the National Academy of Sciences - PNAS. 76 (10), 5061–5065.
Lund, P. A. & Brown, N. L. (1989) Regulation of transcription in Escherichia coli from the mer and merR promoters in the transposon Tn501. Journal of molecular biology. 205 (2), 343–353.
| None |