Part:BBa_K4390038
arsR regulated lambda cI expression
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This is a level 1 part formed by assembly of the following level 0 parts:
| Promoter | K190015 |
| RBS | B0034 |
| N-O-C part | K4390037 |
| Terminator | K4390001 |
Usage and Biology
ArsR is a repressor for arsenical resistance operons in bacteria. In gram-negative bacteria, arsR can bind to the promoter of arsenical resistance operons which repress the expression. This repression activity is controlled by arsenic apperance. When arsenic is appeared in the environment, the arsR will bind to arsenic ions which will no longer be able to bind to the promoter and release the arsenical resistance operons to be expressed (Wu, J. and Rosen, B. P., 1991; Diorio, C. et al., 1995). Lambda cI is a transcriptional repressor which allows Lambda phage to establish and maintain latency after infect E. coli. It regulates the entry of lytic cycle by repressing the lytic promoters (Johnson, A. D. et al., 1979). This Lambda cI sequence was codon optimised for expression in E. coli K12, and was be used in Seamless Enrichment of Ligand-Inducible Sensors (SELIS) as the repressor (d’Oelsnitz, S. et al., 2022).
In this designed part, Lambda cI will be expressed under regulation of arsR, thus Lambda cI will only be expressed when there are arsenic present in the environment. In iGEM22_Edinburgh-UHAS_Ghana SELIS design, the expressed Lambda cI will be used to repress CmR gene which generates chloramphenicol resistance in bacteria. For further information please refer to SELIS arsR evolution construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Diorio, C. et al. (1995) An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria. Journal of Bacteriology. 177 (8), 2050–2056.
D'Oelsnitz, S. et al., (2022) Using fungible biosensors to evolve improved alkaloid biosyntheses. Nature chemical biology. 18 (9), 981–989.
Johnson, A. D. et al. (1979) Interactions between DNA-Bound Repressors Govern Regulation by the $\lambda $ Phage Repressor. Proceedings of the National Academy of Sciences - PNAS. 76 (10), 5061–5065.
Wu, J. & Rosen, B. P. (1991) The ArsR protein is a trans‐acting regulatory protein. Molecular microbiology. 5 (6), 1331–1336.
| None |