Coding
Part:BBa_K4188007
Designed by: Tobias Bröker Group: iGEM22_WWU_Muenster (2022-09-30)
ScERG8 fused to PTS1 tag for the peroxisomal expression
This part is part of the genes needed to replicate the mevalonate pathway in the peroxisome. For this purpose, all required genes have been assembled and can be found in the table below. The gene is MoClo (RFC1000) compatible. The ScERG8 catalyzes the reaction of mevalonate-5-phosphate to mevalonate-5-pyrophosphate.
Genes for the mevalonate pathway in the peroxisome
part number | Gene | length |
---|---|---|
BBa_K4188007 | ScERG8_PTS1 | 1365 bp |
BBa_K4188008 | ScERG19_PTS1 | 1200 bp |
BBa_K4188009 | ScERG13_PTS1 | 1485 bp |
BBa_K4188010 | ScERG10_PTS1 | 1206 bp |
BBa_K4188011 | ScIDI1_PTS1 | 876 bp |
BBa_K4188012 | ScERG12_PTS1 | 1341 bp |
BBa_K4188013 | SltNPPS1_PTS1 | 792 bp |
BBa_K4188014 | AgtGPPS2_PTS1 | 900 bp |
BBa_K4188015 | SctHMGR_PTS1 | 1518 bp |
BBa_K4188016 | GgmFPS144_PTS1 | 1113 bp |
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 919
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 919
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 919
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |