Coding

Part:BBa_K4188015

Designed by: Tobias Bröker   Group: iGEM22_WWU_Muenster   (2022-09-30)


SctHMGR fused to PTS1 tag for the peroxisomal expression

This part is part of the genes needed to replicate the mevalonate pathway in the peroxisome. For this purpose, all required genes have been assembled and can be found in the table below. The gene is MoClo (RFC1000) compatible. The SctHMGR removes the limiting step in the mevalonate pathway the conversion from HMG-CoA to mevalonate. Truncated version without repression side.


Genes for the mevalonate pathway in the peroxisome

part number Gene length
BBa_K4188007 ScERG8_PTS1 1365 bp
BBa_K4188008 ScERG19_PTS1 1200 bp
BBa_K4188009 ScERG13_PTS1 1485 bp
BBa_K4188010 ScERG10_PTS1 1206 bp
BBa_K4188011 ScIDI1_PTS1 876 bp
BBa_K4188012 ScERG12_PTS1 1341 bp
BBa_K4188013 SltNPPS1_PTS1 792 bp
BBa_K4188014 AgtGPPS2_PTS1 900 bp
BBa_K4188015 SctHMGR_PTS1 1518 bp
BBa_K4188016 GgmFPS144_PTS1 1113 bp


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1236
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1236
    Illegal NgoMIV site found at 1381
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None