Part:BBa_K3766033

Synthetic RBS designed for mCherry (reverse orientation)

This part is a synthetic RBS (in reverse orientation) designed for mCherry (BBa_K3766013) using the Salis Lab / De Novo DNA RBS Library Calculator v2.1.1 [1, 2, 3].

Usage and Biology

This RBS was designed for and used to drive the expression of mCherry (BBa_K3766013) under the control of the T7 CGG promoter (BBa_K3766022) promoter in the composite part BBa_K3766111.

It was obtained upon cloning from a library of 16 potential RBS sequences (AAAGCGGTNCAAKMGGAGGTAGAA) having a predicted translation initiation rate in the RBS library of minimum 43.17 and maximum 26447.95 (using the RBS Calculator's proportional scale).

References

[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.

[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]