Synthetic RBS designed for mCherry (reverse orientation)
This part is a synthetic RBS (in reverse orientation) designed for mCherry (BBa_K3766013) using the Salis Lab / De Novo DNA RBS Library Calculator v2.1.1 [1, 2, 3].
Usage and Biology
It was obtained upon cloning from a library of 16 potential RBS sequences (AAAGCGGTNCAAKMGGAGGTAGAA) having a predicted translation initiation rate in the RBS library of minimum 43.17 and maximum 26447.95 (using the RBS Calculator's proportional scale).
 Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
 Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
 Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC