T7 CGG promoter (reverse orientation)
This part is the T7CGG promoter (TAATACCGGTCACTATA) in reverse orientation. It is a variant of the strong promoter from T7 bacteriophage (taatacgactcactata) with GG at 3' end.
Usage and Biology
The T7CGG promoter is recognized specifically by the orthogonal T7 CGG-R12-KIRV RNA polymerase (BBa_K3766000) .
The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GG is one of the best +1, +2 base combinations at the transcription initiation for enhanced promoter strength .
 Meyer AJ, Ellefson JW, Ellington AD. Directed evolution of a panel of orthogonal T7 RNA Polymerase variants for in vivo or in vitro synthetic circuitry. ACS synthetic biology (2015) 4: 1070–1076.
 Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 10
- 1000COMPATIBLE WITH RFC