Measurement

Part:BBa_K299004

Designed by: Anna Olchowik   Group: iGEM10_Warsaw   (2010-07-19)

RBS measurement device J23100 J61127 GFP

This part is licensed under
Creative BioCommons

This device was used to determine BBa_J61127 strength relative to BBa_B0034.

Authors

Clonning:

  • Ania Puławska
  • Ania Safray
  • Milena Bażlekowa

The lab work was supervised by:

  • Jarek Pankowski
  • Ania Olchowik
  • Kasia Grześ

Flow cytometer measurements done by Cherry Moreno

Construct design

The device consists of BBa_J23100 promoter followed by BBa_J61127 Anderson's RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein.

Measurements

Using this construct we have determined that BBa_J61127 strength is 9,63% relative to BBa_B0034

All our measurement results are summarized on the chart below:

RBS_relative_strength.png

For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


[edit]
Categories
Parameters
n/aRBS measurement device J23100 J61127 GFP