Composite

Part:BBa_K2656113

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-30)

amilCP transcriptional unit 1

This is a transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part of our BioArt DNA toolkit palette: Printone , as it can be used to express a blue color with strong intensity.

This transcriptional unit is composed of the following standardized parts from our Part Collection:

  • BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
  • BBa_K2656009: the B0030 very weak ribosome biding site in its Golden Braid compatible version
  • BBa_K2656013: the BBa_K2656018 amilCP blue chromoprotein sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

The characterization of the amilCP protein (BBa_K2656018 blue chromoprotein sequence), and by extension of all the other parts that codify for the amilCP gene, was performed using this transcriptional unit. The absorbance spectrum of the amilCP protein was obtained by using this protocol and MATlab script execution.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None