Composite

Part:BBa_K2656112

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-23)

YFP transcriptional unit 1

Transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part of our BioArt DNA toolkit palette: Printone , as it can be used to express a yellow fluorescent color with medium intensity.

It has the following basic parts:

This composite part was used to perform an spectrum experiment to obtain the excitation and emission spectra of the YFP_LVA tag.

It was also used to test the effect of the degradation tag, performing an experiment with which we measured the increase in protein degradation due to this tag. To do so, we assembled two composite parts, BBa_K2656112 (YFP_LVA tag) and BBa_K2656111 (YFP), with the same promoter, RBS and terminator. The results are further explained in our Improve page

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None