YFP_LVA transcriptional unit 1
Transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
This composite part of our BioArt DNA toolkit palette: Printone , as it can be used to express a yellow fluorescent color with degraded intensity due to the LVA tag.
It has the following basic parts:
This transcriptional unit was used to test the effect of the degradation tag, performing an experiment with which we measured the increase in protein degradation due to this LVA tag. To do so, we assembled two composite parts, BBa_K2656112 (YFP_LVA tag) and BBa_K2656111 (YFP), with the same promoter, RBS and terminator. The results are further explained in our Improve page
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 11
Illegal NheI site found at 34
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC