Composite

Part:BBa_K2656105

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-22)

GFP transcriptional unit 1

Transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

It is also a composite part of our BioArt DNA toolkit palette: Printone , as it can be used to express a green color with medium intensity.

This transcriptional unit is composed of the following standardized parts from our Part Collection:

This transcriptional unit has been used to characterize the constitutive BBa_K2656004 promoter. Following this experimental protocol, we have obtained the parameters to valide our constitutive model and so the results of the next graph have been obtained after an optimization and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.

Promoter experiment.
Figure 1. Promoter expression experiment with composite parts BBa_K2656105, BBa_K2656106 and BBa_K2656107
Table 1. Optimized parameters for the BBa_K2656105.
Parameter Value
Constitutive transcription rate CR CR = 171.15 min-1
Dilution rate μ μ = 0.0058 min-1


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None