Coding

Part:BBa_K2082208

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-07)


Fusion protein Gal4:RpoZ

Gal4:RpoZ fusion protein- BBa_K2082208

This BioBrick contains a fusion protein of the regulatory protein Gal4 of Saccharomyces cerevisae and the omega subunit of the RNA polymerase I of E. coli. Together with the part BBa_K2082227 it can be used as a positive control for the bacterial two-hybrid system. The interaction of the Gal4 protein with Gal11P is well documented (Jeong et al. 2001). Due to the fact that we had some time issues at the end, the functionality of this positive control could not be tested. The generator of this fusion protein is the BioBrick BBa_K2082226

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None