Generator

Part:BBa_K2082227

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


Fusion protein Gal11P:cI (434) generator

Gal11P:cI(434) fusion protein generator- BBa_K2082227

This BioBrick contains a fusion protein of the regulatory protein Gal11P of Saccharomyces cerevisae and the repressor protein cI of the phage 434. Together with the part BBa_K2082226 it can be used as a positive control for the bacterial two-hybrid system. The interaction of the Gal4 protein with Gal11P is well documented (Jeong et al. 2001). Due to the fact that we had some time issues at the end, the functionality of this positive control could not be tested. This is the generator of the fusion protein BBa_K2082209

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None