Generator

Part:BBa_K2082226

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


Fusion protein Gal4:RpoZ generator

Gal4:RpoZ fusion protein generator- BBa_K2082226

This BioBrick contains a fusion protein of the regulatory protein Gal4 of Saccharomyces cerevisae and the omega subunit of the RNA polymerase I of E. coli. Together with the part BBa_K2082227 it can be used as a positive control for the bacterial two-hybrid system. The interaction of the Gal4 protein with Gal11P is well documented (Jeong et al. 2001). Due to the fact that we had some time issues at the end, the functionality of this positive control could not be tested. This is the generator of the fusion protein BBa_K2082208

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 418
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None