Translational_Unit

Part:BBa_K1602014

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-16)

B0034-yagE

This composite represents the 2-dehydro-3-deoxy-D-pentonate aldolases yagE plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602002) (BBa_K1602015). (1)

  • yagE
  • RBS-B0034
https://static.igem.org/mediawiki/parts/8/8e/TU_Darmstadt_plasmid-card_B0034-yagE.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 193


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.

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Categories
Parameters
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