Translational_Unit
RBS-yjhG

Part:BBa_K1602002

Designed by: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-08-28)

B0034-yjhG

This composite represents the D-xylonic acid dehydratases yjhG plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602014) (BBa_K1602015). (1)

  • yjhG
  • RBS-B0034
https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1921
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.

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