Generator
PchA/PchB

Part:BBa_J45319

Designed by: André Green II   Group: iGEM2006_MIT   (2006-10-28)

PchA & PchB enzyme generator

BBa_J45319 takes as input a transcriptional signal (PoPS) and produce as output the PchA and PchB enzymes that catalyzes production of salicylate from the cellular metabolite chorismate. Salicylic acid is the precursor to wintergreen odor.

Usage and Biology

See BBa_J45017 for additional details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 730
    Illegal NgoMIV site found at 855
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_J45319 parameters

Burden Imposed by this Part:

Burden Value: 0.4 ± 2.9%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

[edit]
Categories
//classic/generator/rct
s
Parameters
o_h
proteinPchA & PchB
rbs
tagNone