Generator

Part:BBa_J107051

Designed by: Giacomo Zambianchi   Group: Magni_UNIPV_Lab   (2010-06-01)


Constitutive generator of Beta-Galactosidase + J23100

This part consitutively expresses lacZ (BBa_I732005) under the control of Ptet strong promoter (BBa_R0040), which is always active because tetR gene (BBa_C0040) does not have any promoter upstream and so it cannot repress BBa_R0040.

If this part is contained in BBa_J61002 vector, RFP (BBa_E1010) is constitutively expressed by BBa_J23100 strong promoter.

This part confers the abilities of metabolizing lactose to lacZ mutant strains (e.g. TOP10 - BBa_V1009) and of glowing (when the vector is BBa_J61002), in order to (indirectly) detect bacterial growth by measuring RFP when the medium is turbid and the optical density (OD) cannot be effectively used as a cell count measure.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4155
    Illegal NheI site found at 4178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None