Constitutive generator of Beta-Galactosidase + J23100
This part consitutively expresses lacZ (BBa_I732005) under the control of Ptet strong promoter (BBa_R0040), which is always active because tetR gene (BBa_C0040) does not have any promoter upstream and so it cannot repress BBa_R0040.
This part confers the abilities of metabolizing lactose to lacZ mutant strains (e.g. TOP10 - BBa_V1009) and of glowing (when the vector is BBa_J61002), in order to (indirectly) detect bacterial growth by measuring RFP when the medium is turbid and the optical density (OD) cannot be effectively used as a cell count measure.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 4155
Illegal NheI site found at 4178
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC