Generator

Part:BBa_J107050

Designed by: Giacomo Zambianchi   Group: Magni_UNIPV_Lab   (2010-06-01)

Constitutive generator of Beta-Galactosidase and GFP

This part consitutively expresses lacZ (BBa_I732005) under the control of Ptet strong promoter (BBa_R0040), which is always active because tetR gene (BBa_C0040) does not have any promoter upstream and so it cannot repress BBa_R0040.

GFP (BBa_E0040) is constitutively expressed by another strong promoter: BBa_J23100.

This part confers the abilities of metabolizing lactose to lacZ mutant strains (e.g. TOP10 - BBa_V1009) and of glowing, in order to (indirectly) detect bacterial growth by measuring GFP when the medium is turbid and the optical density (OD) cannot be effectively used as a cell count measure.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4155
    Illegal NheI site found at 4178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4854


[edit]
Categories
Parameters
n/aConstitutive generator of Beta-Galactosidase and GFP