Part:BBa_I739016

Double regulated polycistronic cI +LVA / ECFP +LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged cI (BBa_C0051) and LVA-tagged ECFP (BBa_E0022) under control of the double promoter BBa_I739103. The ribosome binding sites BBa_B0034 are located upstream of each coding region. The transcription of cI and ECFP is terminated by the double terminator BBa_B0015.

Mode of Action

cI production can be repressed by lacI and/or P22 cII. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of P22 cII.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of Part 8 and Part 9.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]